4AS4

Structure of human inositol monophosphatase 1

4AS4 の概要

• エントリーDOI: 10.2210/pdb4as4/pdb
• 関連するPDBエントリー: 1AWB 1IMA 1IMB 1IMC 1IMD 1IME 1IMF 2HHM 4AS5
• 分子名称: INOSITOL MONOPHOSPHATASE 1, PHOSPHATE ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: hydrolase, lithium, bipolar disorder
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm: P29218
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61419.99

構造登録者

Singh, N.,Knight, M.,Halliday, A.C.,Lack, N.A.,Lowe, E.D.,Churchill, G.C. (登録日: 2012-04-27, 公開日: 2012-10-10, 最終更新日: 2024-11-13)

主引用文献

Singh, N.,Halliday, A.C.,Knight, M.,Lack, N.A.,Lowe, E.D.,Churchill, G.C.
Cloning, Expression, Purification, Crystallization and X-Ray Analysis of Inositol Monophosphatase from Mus Musculus and Homo Sapiens.
Acta Crystallogr.,Sect.F, 68:1149-, 2012

PubMed Abstract: Inositol monophosphatase (IMPase) catalyses the hydrolysis of inositol monophosphate to inositol and is crucial in the phosphatidylinositol (PI) signalling pathway. Lithium, which is the drug of choice for bipolar disorder, inhibits IMPase at therapeutically relevant plasma concentrations. Both mouse IMPase 1 (MmIMPase 1) and human IMPase 1 (HsIMPase 1) were cloned into pRSET5a, expressed in Escherichia coli, purified and crystallized using the sitting-drop method. The structures were solved at resolutions of 2.4 and 1.7 Å, respectively. Comparison of MmIMPase 1 and HsIMPase 1 revealed a core r.m.s. deviation of 0.516 Å.

PubMed: 23027737
DOI: 10.1107/S1744309112035191

実験手法

X-RAY DIFFRACTION (1.7 Å)