4ARS

Hafnia Alvei phytase apo form

4ARS の概要

• エントリーDOI: 10.2210/pdb4ars/pdb
• 関連するPDBエントリー: 4ARO 4ARU
• 分子名称: HISTIDINE ACID PHOSPHATASE, GLYCEROL, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: HAFNIA ALVEI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46335.31

構造登録者

Ariza, A.,Moroz, O.V.,Blagova, E.B.,Turkenburg, J.P.,Vevodova, J.,Roberts, S.,Vind, J.,Sjoholm, C.,Lassen, S.F.,De Maria, L.,Glitsoe, V.,Skov, L.K.,Wilson, K.S. (登録日: 2012-04-26, 公開日: 2013-05-08, 最終更新日: 2024-10-23)

主引用文献

Ariza, A.,Moroz, O.V.,Blagova, E.V.,Turkenburg, J.P.,Waterman, J.,Roberts, S.M.,Vind, J.,Sjoholm, C.,Lassen, S.F.,De Maria, L.,Glitsoe, V.,Skov, L.K.,Wilson, K.S.
Degradation of Phytate by the 6-Phytase from Hafnia Alvei: A Combined Structural and Solution Study.
Plos One, 8:65062-, 2013

PubMed Abstract: Phytases hydrolyse phytate (myo-inositol hexakisphosphate), the principal form of phosphate stored in plant seeds to produce phosphate and lower phosphorylated myo-inositols. They are used extensively in the feed industry, and have been characterised biochemically and structurally with a number of structures in the PDB. They are divided into four distinct families: histidine acid phosphatases (HAP), β-propeller phytases, cysteine phosphatases and purple acid phosphatases and also split into three enzyme classes, the 3-, 5- and 6-phytases, depending on the position of the first phosphate in the inositol ring to be removed. We report identification, cloning, purification and 3D structures of 6-phytases from two bacteria, Hafnia alvei and Yersinia kristensenii, together with their pH optima, thermal stability, and degradation profiles for phytate. An important result is the structure of the H. alvei enzyme in complex with the substrate analogue myo-inositol hexakissulphate. In contrast to the only previous structure of a ligand-bound 6-phytase, where the 3-phosphate was unexpectedly in the catalytic site, in the H. alvei complex the expected scissile 6-phosphate (sulphate in the inhibitor) is placed in the catalytic site.

PubMed: 23741456
DOI: 10.1371/JOURNAL.PONE.0065062

実験手法

X-RAY DIFFRACTION (1.9 Å)