4ARR

Crystal structure of the N-terminal domain of Drosophila Toll receptor with the magic triangle I3C

4ARR の概要

• エントリーDOI: 10.2210/pdb4arr/pdb
• 関連するPDBエントリー: 4ARN
• 分子名称: TOLL RECEPTOR, VARIABLE LYMPHOCYTE RECEPTOR B.61 CHIMERA, 2-acetamido-2-deoxy-beta-D-glucopyranose, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid (3 entities in total)
• 機能のキーワード: immune system, cytokine receptor, embryonic development, innate immunity, leucine-rich repeat, lrr hybrid technology
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65696.97

構造登録者

Gangloff, M.,Gay, N.J. (登録日: 2012-04-26, 公開日: 2013-02-27, 最終更新日: 2024-11-13)

主引用文献

Gangloff, M.,Moreno, A.,Gay, N.J.
Liesegang-Like Patterns of Toll Crystals Grown in Gel.
J.Appl.Crystallogr., 46:337-, 2013

PubMed Abstract: Generating high-quality crystals remains a bottleneck in biological and materials sciences. Here a counter-diffusion method was used to improve the X-ray diffraction quality of the N-terminal domain of Toll receptor crystals. It was observed that crystallization occurred with a peculiar pattern along the capillary resembling Liesegang bands; this phenomenon is described at both macroscopic and atomic levels. It was found that bands appeared for native protein as well as for co-crystals of magic triangle (I3C)-bound protein even though they crystallize in different space groups. Crystallization occurred with a linear recurrence independent of the precipitant concentration and a protein-specific spacing coefficient. Bandwidth varied along the capillary, oscillating between large precipitation areas and single crystals. The reported data suggest that repetitive patterns can be generated with biological macromolecules in the presence of sodium malonate as a crystallization agent. A comparison with typical Liesegang patterns and the possible mechanism underlying this phenomenon are discussed.

PubMed: 23596340
DOI: 10.1107/S0021889812051606

実験手法

X-RAY DIFFRACTION (3 Å)