4AMF

Pseudomonas fluorescens PhoX in complex with the substrate analogue AppCp

4AMF の概要

• エントリーDOI: 10.2210/pdb4amf/pdb
• 関連するPDBエントリー: 3ZWU 4A9V 4A9X 4ALF
• 分子名称: PHOX, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, CHLORIDE ION, ... (8 entities in total)
• 機能のキーワード: hydrolase, alkaline phosphatase, phox, beta- propeller, iron, appcp, substrate analogue, phosphomethylphosphonic acid adenylate ester
• 由来する生物種: PSEUDOMONAS FLUORESCENS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 131221.13

構造登録者

Yong, S.C.,Roversi, P.,Lillington, J.E.D.,Zeldin, O.B.,Garman, E.F.,Lea, S.M.,Berks, B.C. (登録日: 2012-03-09, 公開日: 2013-03-20, 最終更新日: 2023-12-20)

主引用文献

Yong, S.C.,Roversi, P.,Lillington, J.,Rodriguez, F.,Krehenbrink, M.,Zeldin, O.B.,Garman, E.F.,Lea, S.M.,Berks, B.C.
A Complex Iron-Calcium Cofactor Catalyzing Phosphotransfer Chemistry
Science, 345:1170-, 2014

PubMed Abstract: Alkaline phosphatases play a crucial role in phosphate acquisition by microorganisms. To expand our understanding of catalysis by this class of enzymes, we have determined the structure of the widely occurring microbial alkaline phosphatase PhoX. The enzyme contains a complex active-site cofactor comprising two antiferromagnetically coupled ferric iron ions (Fe(3+)), three calcium ions (Ca(2+)), and an oxo group bridging three of the metal ions. Notably, the main part of the cofactor resembles synthetic oxide-centered triangular metal complexes. Structures of PhoX-ligand complexes reveal how the active-site metal ions bind substrate and implicate the cofactor oxo group in the catalytic mechanism. The presence of iron in PhoX raises the possibility that iron bioavailability limits microbial phosphate acquisition.

PubMed: 25190793
DOI: 10.1126/SCIENCE.1254237

実験手法

X-RAY DIFFRACTION (1.52 Å)