4ALO

STRUCTURE AND PROPERTIES OF H1 CRUSTACYANIN FROM LOBSTER HOMARUS AMERICANUS

4ALO の概要

• エントリーDOI: 10.2210/pdb4alo/pdb
• 関連するPDBエントリー: 1H91 1I4U 1OBQ 1OBU 1S2P
• 分子名称: H1 APOCRUSTACYANIN, (4S)-2-METHYL-2,4-PENTANEDIOL, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: transport protein, chromophore binding protein, bathochromic shift, astaxanthin, colouration, recombinant carotenoproteins, carapace
• 由来する生物種: HOMARUS AMERICANUS (AMERICAN LOBSTER)
• 細胞内の位置: Secreted, extracellular space: P80029
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42528.63

構造登録者

Ferrari, M.,Folli, C.,Pincolini, E.,Mcclintock, T.S.,Roessle, M.,Berni, R.,Cianci, M. (登録日: 2012-03-05, 公開日: 2012-08-08, 最終更新日: 2024-11-20)

主引用文献

Ferrari, M.,Folli, C.,Pincolini, E.,Mcclintock, T.S.,Rossle, M.,Berni, R.,Cianci, M.
Structural Characterization of Recombinant Crustacyanin Subunits from the Lobster Homarus Americanus.
Acta Crystallogr.,Sect.F, 68:846-, 2012

PubMed Abstract: Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H(1) and H(2) from the American lobster Homarus americanus are reported. The two subunits are structurally highly similar to the corresponding natural apo crustacyanin CRTC and CRTA subunits from the European lobster H. gammarus. Reconstitution studies of the recombinant crustacyanin proteins H(1) and H(2) with astaxanthin reproduced the bathochromic shift of 85-95 nm typical of the natural crustacyanin subunits from H. gammarus in complex with astaxanthin. Moreover, correlations between the presence of crustacyanin genes in crustacean species and the resulting carapace colours with the spectral properties of the subunits in complex with astaxanthin confirmed this genotype-phenotype linkage.

PubMed: 22869108
DOI: 10.1107/S1744309112026103

実験手法

X-RAY DIFFRACTION (2.37 Å)