4AKS

PatG macrocyclase domain

Summary for 4AKS

• Entry DOI: 10.2210/pdb4aks/pdb
• Related: 4AKT
• Descriptor: THIAZOLINE OXIDASE/SUBTILISIN-LIKE PROTEASE (2 entities in total)
• Functional Keywords: hydrolase, patellamide
• Biological source: PROCHLORON DIDEMNI
• Total number of polymer chains: 2
• Total formula weight: 77148.89

Authors

Koehnke, J.,Bent, A.,Houssen, W.E.,Zollman, D.,Morawitz, F.,Shirran, S.,Vendome, J.,Nneoyiegbe, A.F.,Trembleau, L.,Botting, C.H.,Smith, M.C.M.,Jaspars, M.,Naismith, J.H. (deposition date: 2012-02-28, release date: 2012-07-18, Last modification date: 2024-10-23)

Primary citation

Koehnke, J.,Bent, A.,Houssen, W.E.,Zollman, D.,Morawitz, F.,Shirran, S.,Vendome, J.,Nneoyiegbe, A.F.,Trembleau, L.,Botting, C.H.,Smith, M.C.,Jaspars, M.,Naismith, J.H.
The Mechanism of Patellamide Macrocyclization Revealed by the Characterization of the Patg Macrocyclase Domain.
Nat.Struct.Mol.Biol., 19:767-, 2012

PubMed Abstract: Peptide macrocycles are found in many biologically active natural products. Their versatility, resistance to proteolysis and ability to traverse membranes has made them desirable molecules. Although technologies exist to synthesize such compounds, the full extent of diversity found among natural macrocycles has yet to be achieved synthetically. Cyanobactins are ribosomal peptide macrocycles encompassing an extraordinarily diverse range of ring sizes, amino acids and chemical modifications. We report the structure, biochemical characterization and initial engineering of the PatG macrocyclase domain of Prochloron sp. from the patellamide pathway that catalyzes the macrocyclization of linear peptides. The enzyme contains insertions in the subtilisin fold to allow it to recognize a three-residue signature, bind substrate in a preorganized and unusual conformation, shield an acyl-enzyme intermediate from water and catalyze peptide bond formation. The ability to macrocyclize a broad range of nonactivated substrates has wide biotechnology applications.

PubMed: 22796963
DOI: 10.1038/NSMB.2340

Experimental method

X-RAY DIFFRACTION (2.19 Å)