4AIV

Crystal Structure of putative NADH-dependent nitrite reductase small subunit from Mycobacterium tuberculosis

4AIV の概要

• エントリーDOI: 10.2210/pdb4aiv/pdb
• 分子名称: PROBABLE NITRITE REDUCTASE [NAD(P)H] SMALL SUBUNIT NIRD, GLYCEROL (3 entities in total)
• 機能のキーワード: oxidoreductase, nitrite metabolism
• 由来する生物種: MYCOBACTERIUM TUBERCULOSIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12591.31

構造登録者

Izumi, A.,Schnell, R.,Schneider, G. (登録日: 2012-02-15, 公開日: 2012-09-12, 最終更新日: 2023-12-20)

主引用文献

Izumi, A.,Schnell, R.,Schneider, G.
Crystal Structure of Nird, the Small Subunit of the Nitrite Reductase Nirbd from Mycobacterium Tuberculosis, at 2.0 Angstrom Resolution
Proteins, 80:2799-, 2012

PubMed Abstract: NirD is part of the nitrite reductase complex NirBD that catalyses the reduction of nitrite to NH(3) in nitrate assimilation and anaerobic respiration. The crystal structure analysis of NirD from Mycobacterium tuberculosis shows a double β-sandwich fold. NirD is related in three-dimensional structure and sequence to the Rieske proteins; however, it does not contain any Fe-S cluster or other cofactors that might be involved in electron transfer. A cysteine residue at the protein surface, conserved in NirD homologues lacking the iron-sulfur cluster might be important for the interaction with NirB and possibly stabilize one of the Fe-S centers in this subunit.

PubMed: 22965870
DOI: 10.1002/PROT.24177

実験手法

X-RAY DIFFRACTION (2 Å)