4AGF

MTSSL spin labeled L124C mutant of MscS in the open form

4AGF の概要

• エントリーDOI: 10.2210/pdb4agf/pdb
• 関連するPDBエントリー: 2VV5 4AGE
• 分子名称: SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL (1 entity in total)
• 機能のキーワード: membrane protein
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein (By similarity): P0C0S2
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 216390.21

構造登録者

Pliotas, C.,Brannigan, E.,Naismith, J.H. (登録日: 2012-01-26, 公開日: 2012-08-15, 最終更新日: 2023-12-20)

主引用文献

Pliotas, C.,Ward, R.,Branigan, E.,Rasmussen, A.,Hagelueken, G.,Huang, H.,Black, S.S.,Booth, I.R.,Schiemann, O.,Naismith, J.H.
Conformational State of the Mscs Mechanosensitive Channel in Solution Revealed by Pulsed Electron-Electron Double Resonance (Peldor) Spectroscopy.
Proc.Natl.Acad.Sci.USA, 109:E2675-, 2012

PubMed Abstract: The heptameric mechanosensitive channel of small conductance (MscS) provides a critical function in Escherichia coli where it opens in response to increased bilayer tension. Three approaches have defined different closed and open structures of the channel, resulting in mutually incompatible models of gating. We have attached spin labels to cysteine mutants on key secondary structural elements specifically chosen to discriminate between the competing models. The resulting pulsed electron-electron double resonance (PELDOR) spectra matched predicted distance distributions for the open crystal structure of MscS. The fit for the predictions by structural models of MscS derived by other techniques was not convincing. The assignment of MscS as open in detergent by PELDOR was unexpected but is supported by two crystal structures of spin-labeled MscS. PELDOR is therefore shown to be a powerful experimental tool to interrogate the conformation of transmembrane regions of integral membrane proteins.

PubMed: 23012406
DOI: 10.1073/PNAS.1202286109

実験手法

X-RAY DIFFRACTION (4.7 Å)