4AFV

THE STRUCTURE OF METACASPASE 2 FROM T. BRUCEI DETERMINED IN THE PRESENCE OF CALCIUM CHLORIDE

4AFV の概要

• エントリーDOI: 10.2210/pdb4afv/pdb
• 関連するPDBエントリー: 4AF8 4AFP 4AFR
• 分子名称: METACASPASE MCA2 (2 entities in total)
• 機能のキーワード: hydrolase, cysteine peptidase, caspase/hemoglobin fold
• 由来する生物種: TRYPANOSOMA BRUCEI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39958.35

構造登録者

McLuskey, K.,Rudolf, J.,Isaacs, N.W.,Coombs, G.H.,Moss, C.X.,Mottram, J.C. (登録日: 2012-01-23, 公開日: 2012-05-02, 最終更新日: 2024-11-13)

主引用文献

Mcluskey, K.,Rudolf, J.,Proto, W.R.,Isaacs, N.W.,Coombs, G.H.,Moss, C.X.,Mottram, J.C.
Crystal Structure of a Trypanosoma Brucei Metacaspase.
Proc.Natl.Acad.Sci.USA, 109:7469-, 2012

PubMed Abstract: Metacaspases are distantly related caspase-family cysteine peptidases implicated in programmed cell death in plants and lower eukaryotes. They differ significantly from caspases because they are calcium-activated, arginine-specific peptidases that do not require processing or dimerization for activity. To elucidate the basis of these differences and to determine the impact they might have on the control of cell death pathways in lower eukaryotes, the previously undescribed crystal structure of a metacaspase, an inactive mutant of metacaspase 2 (MCA2) from Trypanosoma brucei, has been determined to a resolution of 1.4 Å. The structure comprises a core caspase fold, but with an unusual eight-stranded β-sheet that stabilizes the protein as a monomer. Essential aspartic acid residues, in the predicted S1 binding pocket, delineate the arginine-specific substrate specificity. In addition, MCA2 possesses an unusual N terminus, which encircles the protein and traverses the catalytic dyad, with Y31 acting as a gatekeeper residue. The calcium-binding site is defined by samarium coordinated by four aspartic acid residues, whereas calcium binding itself induces an allosteric conformational change that could stabilize the active site in a fashion analogous to subunit processing in caspases. Collectively, these data give insights into the mechanistic basis of substrate specificity and mode of activation of MCA2 and provide a detailed framework for understanding the role of metacaspases in cell death pathways of lower eukaryotes.

PubMed: 22529389
DOI: 10.1073/PNAS.1200885109

実験手法

X-RAY DIFFRACTION (1.5 Å)