4AF2

C61S mutant of thiol peroxidase form E. coli.

4AF2 の概要

• エントリーDOI: 10.2210/pdb4af2/pdb
• 分子名称: THIOL PEROXIDASE (2 entities in total)
• 機能のキーワード: oxidoreductase, inactive mutant, peroxiredoxin
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Periplasm (By similarity): P0A864
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21622.41

構造登録者

Beckham, K.S.H.,Roe, A.J.,Byron, O.,Gabrielsen, M. (登録日: 2012-01-16, 公開日: 2012-05-02, 最終更新日: 2023-12-20)

主引用文献

Beckham, K.S.H.,Byron, O.,Roe, A.J.,Gabrielsen, M.
The Structure of an Orthorhombic Crystal Form of a `Forced Reduced' Thiol Peroxidase Reveals Lattice Formation Aided by the Presence of the Affinity Tag
Acta Crystallogr.,Sect.F, 68:522-, 2012

PubMed Abstract: Thiol peroxidase (Tpx) is an atypical 2-Cys peroxiredoxin, which has been suggested to be important for cell survival and virulence in Gram-negative pathogens. The structure of a catalytically inactive version of this protein in an orthorhombic crystal form has been determined by molecular replacement. Structural alignments revealed that Tpx is conserved. Analysis of the crystal packing shows that the linker region of the affinity tag is important for formation of the crystal lattice.

PubMed: 22691780
DOI: 10.1107/S1744309112011487

実験手法

X-RAY DIFFRACTION (1.97 Å)