4AD8

Crystal structure of a deletion mutant of Deinococcus radiodurans RecN

4AD8 の概要

• エントリーDOI: 10.2210/pdb4ad8/pdb
• 関連するPDBエントリー: 4ABX 4ABY
• 分子名称: DNA REPAIR PROTEIN RECN (1 entity in total)
• 機能のキーワード: dna binding protein, dna repair, atpase domain
• 由来する生物種: DEINOCOCCUS RADIODURANS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55154.64

構造登録者

Pellegrino, S.,Radzimanowski, J.,de Sanctis, D.,McSweeney, S.,Timmins, J. (登録日: 2011-12-22, 公開日: 2012-12-12, 最終更新日: 2023-12-20)

主引用文献

Pellegrino, S.,Radzimanowski, J.,De Sanctis, D.,Erba, E.B.,Mcsweeney, S.,Timmins, J.
Structural and Functional Characterization of an Smc-Like Protein Recn: New Insights Into Double-Strand Break Repair.
Structure, 20:2076-, 2012

PubMed Abstract: Repair of DNA double-strand breaks (DSBs) is essential for cell survival and maintaining genome integrity. DSBs are repaired in a stepwise manner by homologous recombination. Here, we focused on the early steps of DSB repair, including DSB recognition, which is still only poorly understood. In prokaryotes, this process has been proposed to involve the RecN protein, a member of the structural maintenance of chromosome (SMC) protein family, which include key eukaryotic and prokaryotic proteins such as cohesin, condensin, and Rad50. An extensive high- and low-resolution structural analysis of Deinococcus radiodurans RecN using a combination of protein crystallography and small-angle X-ray scattering enabled us to assemble a quasi-atomic model of the entire RecN protein, representing the complete structure of a SMC-like protein. These results, together with a thorough biochemical and mutational study of RecN, allow us to propose a model for the role of RecN in DSB repair.

PubMed: 23085075
DOI: 10.1016/J.STR.2012.09.010

実験手法

X-RAY DIFFRACTION (3.998 Å)