4ACL

3D Structure of DotU from Francisella novicida

4ACL の概要

• エントリーDOI: 10.2210/pdb4acl/pdb
• 関連するPDBエントリー: 4ACK
• 分子名称: TSSL, SODIUM ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: protein transport, t6ss, microbial surface structures, membrane protein, virulence factor
• 由来する生物種: FRANCISELLA NOVICIDA
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51127.71

構造登録者

Robb, C.S.,Nano, F.E.,Boraston, A.B. (登録日: 2011-12-16, 公開日: 2012-04-25, 最終更新日: 2024-05-08)

主引用文献

Robb, C.S.,Nano, F.E.,Boraston, A.B.
The Structure of the Conserved Type Six Secretion Protein Tssl (Dotu) from Francisella Novicida
J.Mol.Biol., 419:277-, 2012

PubMed Abstract: Type six secretion systems (T6SSs) are found in many Gram-negative bacteria and are important for their virulence or their ecological competitiveness. The multicomponent T6SSs are responsible for the translocation of effector molecules into target eukaryotic or prokaryotic cells. The Francisella pathogenicity island encodes a putative T6SS that Francisella novicida requires for intramacrophage growth and virulence during infection of rodents. Here, we present the X-ray crystal structure of the conserved type six secretion component TssL (DotU) from F. novicida. The structure of this protein, which is referred to as Ftn_TssL, revealed an all-α-helical fold that is a unique fusion of two 3-helix bundles. The sequence of Ftn_TssL shows low identity to presumed homologs that are found in most T6SSs. The structure of Ftn_TssL, however, has allowed us to provide bioinformatics evidence that the F. novicida TssL has a fold that is very likely representative for TssL forms from both T6SSs and from the distantly related B subclass of type four secretion systems. A map of sequence conservation on the TssL structure revealed a surface-exposed groove that may represent a functional site on the protein.

PubMed: 22504227
DOI: 10.1016/J.JMB.2012.04.003

実験手法

X-RAY DIFFRACTION (2.49 Å)