4AB6

Regulatory domain structure of NMB2055 (MetR), C103S C106S mutant, a LysR family regulator from N. meningitidis

4AB6 の概要

• エントリーDOI: 10.2210/pdb4ab6/pdb
• 関連するPDBエントリー: 4AB5
• 分子名称: TRANSCRIPTIONAL REGULATOR, LYSR FAMILY, SULFATE ION (3 entities in total)
• 機能のキーワード: transcription factors, transcription
• 由来する生物種: NEISSERIA MENINGITIDIS SEROGROUP B
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49797.00

構造登録者

Sainsbury, S.,Ren, J.,Saunders, N.J.,Stuart, D.I.,Owens, R.J. (登録日: 2011-12-07, 公開日: 2012-07-11, 最終更新日: 2024-05-08)

主引用文献

Sainsbury, S.,Ren, J.,Saunders, N.J.,Stuart, D.I.,Owens, R.J.
Structure of the Regulatory Domain of the Lysr Family Regulator Nmb2055 (Metr-Like Protein) from Neisseria Meningitidis
Acta Crystallogr.,Sect.F, 68:730-, 2012

PubMed Abstract: The crystal structure of the regulatory domain of NMB2055, a putative MetR regulator from Neisseria meningitidis, is reported at 2.5 Å resolution. The structure revealed that there is a disulfide bond inside the predicted effector-binding pocket of the regulatory domain. Mutation of the cysteines (Cys103 and Cys106) that form the disulfide bond to serines resulted in significant changes to the structure of the effector pocket. Taken together with the high degree of conservation of these cysteine residues within MetR-related transcription factors, it is suggested that the Cys103 and Cys106 residues play an important role in the function of MetR regulators.

PubMed: 22750853
DOI: 10.1107/S1744309112010603

実験手法

X-RAY DIFFRACTION (2.8 Å)