4A5Z

Structures of MITD1

4A5Z の概要

• エントリーDOI: 10.2210/pdb4a5z/pdb
• 関連するPDBエントリー: 4A5X
• 分子名称: MIT DOMAIN-CONTAINING PROTEIN 1, DIMETHYL SULFOXIDE, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: protein transport, escrt, cytokinesis, midbody
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side: Q8WV92
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 79260.40

構造登録者

Hadders, M.A.,Agromayor, M.,Caballe, A.,Obita, T.,Perisic, O.,Williams, R.L.,Martin-Serrano, J. (登録日: 2011-10-30, 公開日: 2012-11-14, 最終更新日: 2024-10-23)

主引用文献

Hadders, M.A.,Agromayor, M.,Obita, T.,Perisic, O.,Caballe, A.,Kloc, M.,Lamers, M.H.,Williams, R.L.,Martin-Serrano, J.
Escrt-III Binding Protein Mitd1 is Involved in Cytokinesis and Has an Unanticipated Pld Fold that Binds Membranes.
Proc.Natl.Acad.Sci.USA, 109:17424-, 2012

PubMed Abstract: The endosomal sorting complexes required for transport (ESCRT) proteins have a critical function in abscission, the final separation of the daughter cells during cytokinesis. Here, we describe the structure and function of a previously uncharacterized ESCRT-III interacting protein, MIT-domain containing protein 1 (MITD1). Crystal structures of MITD1 reveal a dimer, with a microtubule-interacting and trafficking (MIT) domain at the N terminus and a unique, unanticipated phospholipase D-like (PLD) domain at the C terminus that binds membranes. We show that the MIT domain binds to a subset of ESCRT-III subunits and that this interaction mediates MITD1 recruitment to the midbody during cytokinesis. Depletion of MITD1 causes a distinct cytokinetic phenotype consistent with destabilization of the midbody and abscission failure. These results suggest a model whereby MITD1 coordinates the activity of ESCRT-III during abscission with earlier events in the final stages of cell division.

PubMed: 23045692
DOI: 10.1073/PNAS.1206839109

実験手法

X-RAY DIFFRACTION (2.3 Å)