4A2F

Coriolopsis gallica laccase collected at 12.65 keV

4A2F の概要

• エントリーDOI: 10.2210/pdb4a2f/pdb
• 関連するPDBエントリー: 4A2D 4A2E 4A2G 4A2H
• 分子名称: LACCASE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, COPPER (II) ION, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, multicopper oxidase, x-ray induced reduction
• 由来する生物種: CORIOLOPSIS GALLICA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53926.95

構造登録者

De la Mora, E.,Rudino-Pinera, E. (登録日: 2011-09-26, 公開日: 2012-04-25, 最終更新日: 2024-10-16)

主引用文献

De La Mora, E.,Lovett, J.E.,Blanford, C.F.,Garman, E.F.,Valderrama, B.,Rudino-Pinera, E.
Structural Changes Caused by Radiation-Induced Reduction and Radiolysis: The Effect of X-Ray Absorbed Dose in a Fungal Multicopper Oxidase
Acta Crystallogr.,Sect.D, 68:564-, 2012

PubMed Abstract: X-ray radiation induces two main effects at metal centres contained in protein crystals: radiation-induced reduction and radiolysis and a resulting decrease in metal occupancy. In blue multicopper oxidases (BMCOs), the geometry of the active centres and the metal-to-ligand distances change depending on the oxidation states of the Cu atoms, suggesting that these alterations are catalytically relevant to the binding, activation and reduction of O(2). In this work, the X-ray-determined three-dimensional structure of laccase from the basidiomycete Coriolopsis gallica (Cg L), a high catalytic potential BMCO, is described. By combining spectroscopic techniques (UV-Vis, EPR and XAS) and X-ray crystallography, structural changes at and around the active copper centres were related to pH and absorbed X-ray dose (energy deposited per unit mass). Depletion of two of the four active Cu atoms as well as low occupancies of the remaining Cu atoms, together with different conformations of the metal centres, were observed at both acidic pH and high absorbed dose, correlating with more reduced states of the active coppers. These observations provide additional evidence to support the role of flexibility of copper sites during O(2) reduction. This study supports previous observations indicating that interpretations regarding redox state and metal coordination need to take radiation effects explicitly into account.

PubMed: 22525754
DOI: 10.1107/S0907444912005343

実験手法

X-RAY DIFFRACTION (1.9 Å)