4A2A

Thermotoga maritima FtsA:FtsZ(336-351)

4A2A の概要

• エントリーDOI: 10.2210/pdb4a2a/pdb
• 関連するPDBエントリー: 1E4F 1E4G 1W5F 4A2B
• 分子名称: CELL DIVISION PROTEIN FTSA, PUTATIVE, CELL DIVISION PROTEIN FTSZ, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: cell cycle, actin, divisome
• 由来する生物種: THERMOTOGA MARITIMA; 詳細
• 細胞内の位置: Cytoplasm (By similarity): O08398
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98725.86

構造登録者

Szwedziak, P.,Lowe, J. (登録日: 2011-09-23, 公開日: 2012-04-25, 最終更新日: 2023-12-20)

主引用文献

Szwedziak, P.,Wang, Q.,Freund, S.M.V.,Lowe, J.
Ftsa Forms Actin-Like Protofilaments
Embo J., 31:2249-, 2012

PubMed Abstract: FtsA is an early component of the Z-ring, the structure that divides most bacteria, formed by tubulin-like FtsZ. FtsA belongs to the actin family of proteins, showing an unusual subdomain architecture. Here we reconstitute the tethering of FtsZ to the membrane via FtsA's C-terminal amphipathic helix in vitro using Thermotoga maritima proteins. A crystal structure of the FtsA:FtsZ interaction reveals 16 amino acids of the FtsZ tail bound to subdomain 2B of FtsA. The same structure and a second crystal form of FtsA reveal that FtsA forms actin-like protofilaments with a repeat of 48 Å. The identical repeat is observed when FtsA is polymerized using a lipid monolayer surface and FtsAs from three organisms form polymers in cells when overexpressed, as observed by electron cryotomography. Mutants that disrupt polymerization also show an elongated cell division phenotype in a temperature-sensitive FtsA background, demonstrating the importance of filament formation for FtsA's function in the Z-ring.

PubMed: 22473211
DOI: 10.1038/EMBOJ.2012.76

実験手法

X-RAY DIFFRACTION (1.8 Å)