4A0J

Crystal structure of Survivin bound to the phosphorylated N-terminal tail of histone H3

4A0J の概要

• エントリーDOI: 10.2210/pdb4a0j/pdb
• 関連するPDBエントリー: 1E31 1F3H 1XOX 4A0I
• 分子名称: BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5, HISTONE H3 PEPTIDE, ZINC ION, ... (4 entities in total)
• 機能のキーワード: cell cycle, mitosis, chromosomal passenger complex, chromatin
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm: O15392
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34531.86

構造登録者

Jeyaprakash, A.A.,Basquin, C.,Jayachandran, U.,Conti, E. (登録日: 2011-09-09, 公開日: 2011-11-09, 最終更新日: 2024-10-23)

主引用文献

Jeyaprakash, A.A.,Basquin, C.,Jayachandran, U.,Conti, E.
Structural Basis for the Recognition of Phosphorylated Histone H3 by the Survivin Subunit of the Chromosomal Passenger Complex.
Structure, 19:1625-, 2011

PubMed Abstract: Localization of the chromosomal passenger complex (CPC) at centromeres during early mitosis is essential for accurate chromosome segregation and is dependent on the phosphorylation of histone H3. We report the 2.7 Å resolution structure of the CPC subunit Survivin bound to the N-terminal tail of histone H3 carrying the Thr3 phosphorylation mark (Thr3ph). The BIR domain of Survivin recognizes the Ala1-Arg2-Thr3ph-Lys4 sequence, decoding the modification state and the free N terminus of histone H3 by a strategy similar to that used by PHD fingers. The structural analysis permitted the identification of putative Survivin-binding epitopes in other mitotic proteins, including human Shugoshin 1. Using biophysical and structural data, we show that a phospho-mimic N-terminal sequence such as that of hSgo1 (Ala1-Lys2-Glu3-Arg4) contains the specificity determinants to bind Survivin. Our findings suggest that the CPC engages in mutually exclusive interactions with other constituents of the mitotic machinery and a histone mark in chromatin.

PubMed: 22032967
DOI: 10.1016/J.STR.2011.09.002

実験手法

X-RAY DIFFRACTION (2.803 Å)