467D

The structure of a decamer forming a four-way junction

467D の概要

• エントリーDOI: 10.2210/pdb467d/pdb
• 分子名称: DNA (5'-D(*CP*CP*GP*GP*GP*AP*CP*CP*GP*G)-3') (2 entities in total)
• 機能のキーワード: g:a mismatches, four-way-junction, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6142.01

構造登録者

Ortiz-Lombardia, M.,Coll, M. (登録日: 1999-04-22, 公開日: 2000-04-22, 最終更新日: 2023-12-27)

主引用文献

Ortiz-Lombardia, M.,Gonzalez, A.,Eritja, R.,Aymami, J.,Azorin, F.,Coll, M.
Crystal structure of a DNA Holliday junction
Nat.Struct.Biol., 6:913-917, 1999

PubMed Abstract: DNA recombination is a universal biological event responsible both for the generation of genetic diversity and for the maintenance of genome integrity. A four-way DNA junction, also termed Holliday junction, is the key intermediate in nearly all recombination processes. This junction is the substrate of recombination enzymes that promote branch migration or catalyze its resolution. We have determined the crystal structure of a four-way DNA junction by multiwavelength anomalous diffraction, and refined it to 2.16 A resolution. The structure has two-fold symmetry, with pairwise stacking of the double-helical arms, which form two continuous B-DNA helices that run antiparallel, cross in a right-handed way, and contain two G-A mismatches. The exchanging backbones form a compact structure with strong van der Waals contacts and hydrogen bonds, implying that a conformational change must occur for the junction to branch-migrate or isomerize. At the branch point, two phosphate groups from one helix occupy the major groove of the other one, establishing sequence-specific hydrogen bonds. These interactions, together with different stacking energies and steric hindrances, explain the preference for a particular junction stacked conformer.

PubMed: 10504723
DOI: 10.1038/13277

実験手法

X-RAY DIFFRACTION (2.16 Å)