464D

DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX

464D の概要

• エントリーDOI: 10.2210/pdb464d/pdb
• 分子名称: RNA (5'-R(*UP*AP*GP*CP*UP*CP*C)-3'), RNA (5'-R(*GP*GP*GP*GP*CP*(IU)P*A)-3'), STRONTIUM ION, ... (5 entities in total)
• 機能のキーワード: 7 base-pair trna ala acceptor stem, double helix, ribonucleic acid, disordered model, rna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 9229.90

構造登録者

Mueller, U.,Muller, Y.A.,Herbst-Irmer, R.,Sprinzl, M.,Heinemann, U. (登録日: 1999-04-14, 公開日: 1999-08-16, 最終更新日: 2024-02-28)

主引用文献

Mueller, U.,Muller, Y.A.,Herbst-Irmer, R.,Sprinzl, M.,Heinemann, U.
Disorder and twin refinement of RNA heptamer double helices.
Acta Crystallogr.,Sect.D, 55:1405-1413, 1999

PubMed Abstract: An RNA helix with seven base pairs which was derived from the acceptor stem of Escherichia coli tRNA(Ala), rGGGGCUA.rUAGCUCC (ALA(wt)), as well as a variant, rGGGGCUA.rUAGCCCC (ALA(C70)), in which the single G.U wobble base pair of ALA(wt) was replaced by G.C, crystallize in space group C2. Both non-isomorphic crystal forms display a complex packing pattern, which can be described alternatively as disorder or pseudo-merohedral twinning. The structure of ALA(wt) was determined by SIRAS phasing using an isomorphous iodine derivative, rGGGGCi(5)UA.rUAGCUCC (ALA(I)). All three RNA structures were subsequently subjected to twin refinement in space group P1, using anisotropic thermal displacement parameters at resolutions of 1.16, 1.23 and 1.4 A for ALA(wt), ALA(I) and ALA(C70), respectively. Alternatively, the structure of ALA(wt) was refined in space group C2 assuming twofold disorder of the molecular orientation. The refined structures are of reasonable quality according to all available indicators. There are no systematic differences between the molecular models resulting from twin refinement and disorder refinement.

PubMed: 10417408
DOI: 10.1107/S0907444999007441

実験手法

X-RAY DIFFRACTION (1.23 Å)