463D

CALCIUM FORM OF D(CGCGAATTCGCG)2

463D の概要

• エントリーDOI: 10.2210/pdb463d/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3'), CALCIUM ION (3 entities in total)
• 機能のキーワード: ca2+ binding, dickerson-drew dodecamer, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7487.10

構造登録者

Liu, J.,Subirana, J.A. (登録日: 1999-03-29, 公開日: 1999-10-08, 最終更新日: 2024-02-28)

主引用文献

Liu, J.,Subirana, J.A.
Structure of d(CGCGAATTCGCG) in the presence of Ca(2+) ions.
J.Biol.Chem., 274:24749-24752, 1999

PubMed Abstract: The dodecamer d(CGCGAATTCGCG) was the first oligonucleotide to be crystallized as a B-DNA duplex. Its structure was analyzed in detail in the early 1980s. Here we show that, in the presence of Ca(2+), it crystallizes in a different way (R3 space group). The dodecamers form parallel columns of straight duplexes with ten base pairs in the B form. The terminal cytosines in each molecule are disordered, whereas the terminal guanines are placed in the minor groove of neighbor duplexes. The central GAATTC region is practically identical to that found in the classic structure of the same dodecamer crystallized in the P2(1)2(1)2(1) space group in the presence of Mg(2+) and spermine. Its structure is thus independent of the crystallization conditions which have been used.

PubMed: 10455145
DOI: 10.1074/jbc.274.35.24749

実験手法

X-RAY DIFFRACTION (1.45 Å)