462D

CRYSTAL STRUCTURE OF THE HIV-1 GENOMIC RNA DIMERIZATION INITIATION SITE

Summary for 462D

• Entry DOI: 10.2210/pdb462d/pdb
• Descriptor: RNA (5'-R(*CP*UP*UP*GP*CP*UP*GP*AP*GP*GP*UP*GP*CP*AP*CP*AP*CP*AP*GP*CP*AP*AP*G) -3'), MAGNESIUM ION (3 entities in total)
• Functional Keywords: hiv, rna duplex, mismatch, bulges, magnesium binding, rna
• Total number of polymer chains: 2
• Total formula weight: 14999.38

Authors

Ennifar, E.,Yusupov, M.,Walter, P.,Marquet, R.,Ehresmann, C.,Ehresmann, B.,Dumas, P. (deposition date: 1999-03-18, release date: 1999-12-02, Last modification date: 2023-12-27)

Primary citation

Ennifar, E.,Yusupov, M.,Walter, P.,Marquet, R.,Ehresmann, B.,Ehresmann, C.,Dumas, P.
The crystal structure of the dimerization initiation site of genomic HIV-1 RNA reveals an extended duplex with two adenine bulges.
Structure Fold.Des., 7:1439-1449, 1999

PubMed Abstract: An important step in retroviral replication is dimerization of the genomic RNA prior to encapsidation. Dimerization is initiated by the formation of a transient 'kissing-loop complex' that is thought to be subsequently matured into an extended duplex by the nucleocapsid protein (NCp). Although chemical probing and nuclear magnetic resonance spectroscopy have provided insight into the structure of the kissing-loop structure, no structural information concerning the extended-duplex state is available so far.

PubMed: 10574792
DOI: 10.1016/S0969-2126(00)80033-7

Experimental method

X-RAY DIFFRACTION (2.3 Å)