430D

STRUCTURE OF SARCIN/RICIN LOOP FROM RAT 28S RRNA

430D の概要

• エントリーDOI: 10.2210/pdb430d/pdb
• 分子名称: SARCIN/RICIN LOOP FROM RAT 28S R-RNA, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: u-rna, double helix, hairpin, blunt stem, tetraloop mismatched, base triple, ribonucleic acid, rna
• 由来する生物種: Rattus norvegicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9674.32

構造登録者

Correll, C.C.,Munishkin, A.,Chan, Y.L.,Ren, Z.,Wool, I.G.,Steitz, T.A. (登録日: 1998-10-04, 公開日: 1998-10-07, 最終更新日: 2024-02-28)

主引用文献

Correll, C.C.,Munishkin, A.,Chan, Y.L.,Ren, Z.,Wool, I.G.,Steitz, T.A.
Crystal structure of the ribosomal RNA domain essential for binding elongation factors.
Proc.Natl.Acad.Sci.USA, 95:13436-13441, 1998

PubMed Abstract: The structure of a 29-nucleotide RNA containing the sarcin/ricin loop (SRL) of rat 28 S rRNA has been determined at 2.1 A resolution. Recognition of the SRL by elongation factors and by the ribotoxins, sarcin and ricin, requires a nearly universal dodecamer sequence that folds into a G-bulged cross-strand A stack and a GAGA tetraloop. The juxtaposition of these two motifs forms a distorted hairpin structure that allows direct recognition of bases in both grooves as well as recognition of nonhelical backbone geometry and two 5'-unstacked purines. Comparisons with other RNA crystal structures establish the cross-strand A stack and the GNRA tetraloop as defined and modular RNA structural elements. The conserved region at the top is connected to the base of the domain by a region presumed to be flexible because of the sparsity of stabilizing contacts. Although the conformation of the SRL RNA previously determined by NMR spectroscopy is similar to the structure determined by x-ray crystallography, significant differences are observed in the "flexible" region and to a lesser extent in the G-bulged cross-strand A stack.

PubMed: 9811818
DOI: 10.1073/pnas.95.23.13436

実験手法

X-RAY DIFFRACTION (2.1 Å)