413D

A'-FORM RNA DOUBLE HELIX IN THE SINGLE CRYSTAL STRUCTURE OF R(UGAGCUUCGGCUC)

Replaces:  373D

Summary for 413D

• Entry DOI: 10.2210/pdb413d/pdb
• Descriptor: RNA (5'-R(*UP*GP*AP*GP*CP*UP*UP*CP*GP*GP*CP*UP*C)-3') (2 entities in total)
• Functional Keywords: a'-rna, double helix, rna
• Total number of polymer chains: 1
• Total formula weight: 4110.46

Authors

Tanaka, Y.,Fujii, S.,Hiroaki, H.,Sakata, T.,Tanaka, T.,Uesugi, S.,Tomita, K.-I.,Kyogoku, Y. (deposition date: 1998-07-10, release date: 1998-07-10, Last modification date: 2024-02-28)

Primary citation

Tanaka, Y.,Fujii, S.,Hiroaki, H.,Sakata, T.,Tanaka, T.,Uesugi, S.,Tomita, K.,Kyogoku, Y.
A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC).
Nucleic Acids Res., 27:949-955, 1999

PubMed Abstract: Here we demonstrate the presence of the A'-RNA conformation using the single crystal structure of a tridecamer: r(UGAGCUUCGGCUC). The average A'-RNA conformation deduced from X-ray fiber diffraction data had only been available previously, but now the presence of the A'-RNA conformation has been found in a single crystal structure for the first time. Statistical analysis showed that the A'-RNA conformation is distinguishable from the A-RNA conformation in a plot of the major groove width against the base pair inclination angle. The major groove of the A'-RNA conformation is wide enough to accommodate a protein or peptide while that of the A-RNA conformation is too narrow to do so. The presence of the A'-RNA conformation is significant for protein-RNA interaction.

PubMed: 9927725
DOI: 10.1093/nar/27.4.949

Experimental method

X-RAY DIFFRACTION (1.8 Å)