400D

THE INTRINSIC STRUCTURE AND STABILITY OF OUT-OF-ALTERNATION BASE PAIRS IN Z-DNA

400D の概要

• エントリーDOI: 10.2210/pdb400d/pdb
• 分子名称: DNA (5'-D(*(5CM)P*GP*GP*CP*(5CM)P*G)-3') (2 entities in total)
• 機能のキーワード: z-dna double helix, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3676.52

構造登録者

Eichman, B.F.,Basham, B.,Schroth, G.P.,Ho, P.S. (登録日: 1998-05-28, 公開日: 1998-07-09, 最終更新日: 2024-02-28)

主引用文献

Eichman, B.F.,Schroth, G.P.,Basham, B.E.,Ho, P.S.
The intrinsic structure and stability of out-of-alternation base pairs in Z-DNA.
Nucleic Acids Res., 27:543-550, 1999

PubMed Abstract: Alternating pyrimidine-purine sequences typically form Z-DNA, with the pyrimidines in the anti and purines in the syn conformations. The observation that dC and dT nucleotides can also adopt the syn conformation (i.e. the nucleotides are out-of-alternation) extends the range of sequences that can convert to this left-handed form of DNA. Here, we study the effects of placing two adjacent d(G*C) base pairs as opposed to a single d(G*C) base pair or two d(A*T) base pairs out-of-alternation by comparing the structure of d(m5CGGCm5CG)2with the previously published structures of d(m5CGGGm5CG)*d(m5CGCCm5CG) and d(m5CGATm5CG)2. A high buckle and loss of stacking interactions are observed as intrinsic properties of the out-of-alternation base pairs regardless of sequence and the context of the dinucleotide. From solution titrations, we find that the destabilizing effect of out-of-alternation d(G*C) base pairs are identical whether these base pairs are adjacent or isolated. We can therefore conclude that it is these intrinsic distortions in the structure of the base pairs and not neighboring effects that account for the inability of out-of-alternation base pairs to adopt the left-handed Z conformation.

PubMed: 9862978
DOI: 10.1093/nar/27.2.543

実験手法

X-RAY DIFFRACTION (1.65 Å)