3ZZQ

Engineered 12-subunit Bacillus subtilis trp RNA-binding attenuation protein (TRAP)

Summary for 3ZZQ

• Entry DOI: 10.2210/pdb3zzq/pdb
• Related: 1WAP 3ZZL
• Descriptor: TRANSCRIPTION ATTENUATION PROTEIN MTRB, TRYPTOPHAN (3 entities in total)
• Functional Keywords: transcription, transcription regulation, protein engineering
• Biological source: BACILLUS SUBTILIS
• Total number of polymer chains: 6
• Total formula weight: 45297.28

Authors

Chen, C.,Smits, C.,Dodson, G.G.,Shevtsov, M.B.,Merlino, N.,Gollnick, P.,Antson, A.A. (deposition date: 2011-09-02, release date: 2011-10-12, Last modification date: 2023-12-20)

Primary citation

Chen, C.,Smits, C.,Dodson, G.G.,Shevtsov, M.B.,Merlino, N.,Gollnick, P.,Antson, A.A.
How to Change the Oligomeric State of a Circular Protein Assembly: Switch from 11-Subunit to 12-Subunit Trap Suggests a General Mechanism
Plos One, 6:25296-, 2011

PubMed Abstract: Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements. The subunit number is arguably the most critical parameter of a circular protein assembly, affecting the internal and external diameters of the assembly and often impacting on the protein's function. Although accurate structural information has been obtained for several circular proteins, a lack of accurate information on alternative oligomeric states has prevented engineering such transitions. In this study we used the bacterial transcription regulator TRAP as a model system to investigate the features that define the oligomeric state of a circular protein and to question how the subunit number could be manipulated.

PubMed: 21984911
DOI: 10.1371/JOURNAL.PONE.0025296

Experimental method

X-RAY DIFFRACTION (1.75 Å)