3ZXU

Crystal structure of the Ctf19-Mcm21 kinetochore heterodimer from yeast

Summary for 3ZXU

• Entry DOI: 10.2210/pdb3zxu/pdb
• Descriptor: MCM21, CTF19, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: cell cycle, coma complex, protein complex, cell division
• Biological source: KLUYVEROMYCES LACTIS; More
• Total number of polymer chains: 4
• Total formula weight: 129460.93

Authors

Schmitzberger, F.,Harrison, S.C. (deposition date: 2011-08-15, release date: 2012-03-14, Last modification date: 2024-05-08)

Primary citation

Schmitzberger, F.,Harrison, S.C.
Rwd Domain: A Recurring Module in Kinetochore Architecture Shown by a Ctf19-Mcm21 Complex Structure.
Embo Rep., 13:216-, 2012

PubMed Abstract: The proteins Ctf19, Okp1, Mcm21 and Ame1 are the components of COMA, a subassembly of budding-yeast kinetochores. We have determined the crystal structure of a conserved COMA subcomplex--the Ctf19-Mcm21 heterodimer--from Kluyveromyces lactis. Both proteins contain 'double-RWD' domains, which together form a Y-shaped framework with flexible N-terminal extensions. The kinetochore proteins Csm1, Spc24 and Spc25 have related single RWD domains, and Ctf19 and Mcm21 associate with pseudo-twofold symmetry analogous to that in the Csm1 homodimer and the Spc24-Spc25 heterodimer. The double-RWD domain core of the Ctf19-Mcm21 heterodimer is sufficient for association with Okp1-Ame1; the less conserved N-terminal regions may interact with components of a more extensive 'CTF19 complex'. Our structure shows the RWD domain to be a recurring module of kinetochore architecture that may be present in other kinetochore substructures. Like many eukaryotic molecular machines, kinetochores may have evolved from simpler assemblies by multiplication of a few ancestral modules.

PubMed: 22322944
DOI: 10.1038/EMBOR.2012.1

Experimental method

X-RAY DIFFRACTION (3.7 Å)