3ZX8

Cryo-EM reconstruction of native and expanded Turnip Crinkle virus

3ZX8 の概要

• エントリーDOI: 10.2210/pdb3zx8/pdb
• 関連するPDBエントリー: 3ZX9 3ZXA
• EMDBエントリー: 1863 1864
• 分子名称: CAPSID PROTEIN (1 entity in total)
• 機能のキーワード: virus, genomic rna structure, genome uncoating, ssrna virus, icosahedral
• 由来する生物種: TURNIP CRINKLE VIRUS (TCV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 113266.76

構造登録者

Bakker, S.E.,Robottom, J.,Hogle, J.M.,Maeda, A.,Pearson, A.R.,Stockley, P.G.,Ranson, N.A.,Harrison, S.C. (登録日: 2011-08-08, 公開日: 2012-07-18, 最終更新日: 2024-05-08)

主引用文献

Bakker, S.E.,Ford, R.J.,Barker, A.M.,Robottom, J.,Saunders, K.,Pearson, A.R.,Ranson, N.A.,Stockley, P.G.
Isolation of an Asymmetric RNA Uncoating Intermediate for a Single-Stranded RNA Plant Virus.
J.Mol.Biol., 417:65-, 2012

PubMed Abstract: We have determined the three-dimensional structures of both native and expanded forms of turnip crinkle virus (TCV), using cryo-electron microscopy, which allows direct visualization of the encapsidated single-stranded RNA and coat protein (CP) N-terminal regions not seen in the high-resolution X-ray structure of the virion. The expanded form, which is a putative disassembly intermediate during infection, arises from a separation of the capsid-forming domains of the CP subunits. Capsid expansion leads to the formation of pores that could allow exit of the viral RNA. A subset of the CP N-terminal regions becomes proteolytically accessible in the expanded form, although the RNA remains inaccessible to nuclease. Sedimentation velocity assays suggest that the expanded state is metastable and that expansion is not fully reversible. Proteolytically cleaved CP subunits dissociate from the capsid, presumably leading to increased electrostatic repulsion within the viral RNA. Consistent with this idea, electron microscopy images show that proteolysis introduces asymmetry into the TCV capsid and allows initial extrusion of the genome from a defined site. The apparent formation of polysomes in wheat germ extracts suggests that subsequent uncoating is linked to translation. The implication is that the viral RNA and its capsid play multiple roles during primary infections, consistent with ribosome-mediated genome uncoating to avoid host antiviral activity.

PubMed: 22306464
DOI: 10.1016/J.JMB.2012.01.017

実験手法

ELECTRON MICROSCOPY (11.5 Å)