3ZVU

Structure of the PYR1 His60Pro mutant in complex with the HAB1 phosphatase and Abscisic acid

3ZVU の概要

• エントリーDOI: 10.2210/pdb3zvu/pdb
• 分子名称: ABSCISIC ACID RECEPTOR PYR1, PROTEIN PHOSPHATASE 2C 16, (2Z,4E)-5-[(1S)-1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid, ... (6 entities in total)
• 機能のキーワード: receptor-hydrolase complex, hormone receptor, stress response, receptor/hydrolase
• 由来する生物種: ARABIDOPSIS THALIANA (THALE CRESS); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59578.45

構造登録者

Betz, K.,Dupeux, F.,Santiago, J.,Rodriguez, P.L.,Marquez, J.A. (登録日: 2011-07-27, 公開日: 2012-06-13, 最終更新日: 2023-12-20)

主引用文献

Dupeux, F.,Santiago, J.,Betz, K.,Twycross, J.,Park, S.,Rodriguez, L.,Gonzalez-Guzman, M.,Jensen, M.R.,Krasnogor, N.,Blackledge, M.,Holdsworth, M.,Cutler, S.R.,Rodriguez, P.L.,Marquez, J.A.
A Thermodynamic Switch Modulates Abscisic Acid Receptor Sensitivity.
Embo J., 30:4171-, 2011

PubMed Abstract: Abscisic acid (ABA) is a key hormone regulating plant growth, development and the response to biotic and abiotic stress. ABA binding to pyrabactin resistance (PYR)/PYR1-like (PYL)/Regulatory Component of Abscisic acid Receptor (RCAR) intracellular receptors promotes the formation of stable complexes with certain protein phosphatases type 2C (PP2Cs), leading to the activation of ABA signalling. The PYR/PYL/RCAR family contains 14 genes in Arabidopsis and is currently the largest plant hormone receptor family known; however, it is unclear what functional differentiation exists among receptors. Here, we identify two distinct classes of receptors, dimeric and monomeric, with different intrinsic affinities for ABA and whose differential properties are determined by the oligomeric state of their apo forms. Moreover, we find a residue in PYR1, H60, that is variable between family members and plays a key role in determining oligomeric state. In silico modelling of the ABA activation pathway reveals that monomeric receptors have a competitive advantage for binding to ABA and PP2Cs. This work illustrates how receptor oligomerization can modulate hormonal responses and more generally, the sensitivity of a ligand-dependent signalling system.

PubMed: 21847091
DOI: 10.1038/EMBOJ.2011.294

実験手法

X-RAY DIFFRACTION (2.1 Å)