3ZRY

Rotor architecture in the F(1)-c(10)-ring complex of the yeast F-ATP synthase

Summary for 3ZRY

• Entry DOI: 10.2210/pdb3zry/pdb
• Related: 1QO1 2WPD 2XOK
• Descriptor: ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL, ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL, ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL, ... (8 entities in total)
• Functional Keywords: hydrolase, atp-binding, f(1)-f(o)atp synthase, mitochondria, molecular motor, central stalk, membrane protein, c-ring
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Total number of polymer chains: 19
• Total formula weight: 450706.63

Authors

Giraud, M.-F.,Dautant, A. (deposition date: 2011-06-21, release date: 2012-01-18, Last modification date: 2023-12-20)

Primary citation

Giraud, M.-F.,Paumard, P.,Sanchez, C.,Brethes, D.,Velours, J.,Dautant, A.
Rotor Architecture in the Yeast and Bovine F(1)-C-Ring Complexes of F-ATP Synthase.
J.Struct.Biol., 177:490-, 2012

PubMed Abstract: The F(1)F(O)-ATP synthase is a rotary molecular nanomotor. F(1) is a chemical motor driven by ATP hydrolysis while F(O) is an electrical motor driven by the proton flow. The two stepping motors are mechanically coupled through a common rotary shaft. Up to now, the three available crystal structures of the F(1)c(10) sub-complex of the yeast F(1)F(O)-ATP synthase were isomorphous and then named yF(1)c(10)(I). In this crystal form, significant interactions of the c(10)-ring with the F(1)-head of neighboring molecules affected the overall conformation of the F(1)-c-ring complex. The symmetry axis of the F(1)-head and the inertia axis of the c-ring were tilted near the interface between the F(1)-central stalk and the c-ring rotor, resulting in an unbalanced machine. We have solved a new crystal form of the F(1)c(10) complex, named yF(1)c(10)(II), inhibited by adenylyl-imidodiphosphate (AMP-PNP) and dicyclohexylcarbodiimide (DCCD), at 6.5Å resolution in which the crystal packing has a weaker influence over the conformation of the F(1)-c-ring complex. yF(1)c(10)(II) provides a model of a more efficient generator. yF(1)c(10)(II) and bovine bF(1)c(8) structures share a common rotor architecture with the inertia center of the F(1)-stator close to the rotor axis.

PubMed: 22119846
DOI: 10.1016/J.JSB.2011.10.015

Experimental method

X-RAY DIFFRACTION (6.5 Å)