3ZPF

Structure of the carboxy-terminal domain of the turkey type 3 siadenovirus fibre

3ZPF の概要

• エントリーDOI: 10.2210/pdb3zpf/pdb
• 関連するPDBエントリー: 3ZPE
• 分子名称: FIBER, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: viral protein, turkey hemorrhagic enteritis virus, beta-sandwich
• 由来する生物種: AVIRULENT TURKEY HEMORRHAGIC ENTERITIS VIRUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21447.21

構造登録者

Singh, A.K.,van Raaij, M.J. (登録日: 2013-02-27, 公開日: 2014-03-12, 最終更新日: 2024-10-16)

主引用文献

Singh, A.K.,Berbis, M.A.,Ballmann, M.Z.,Kilcoyne, M.,Menendez, M.,Nguyen, T.H.,Joshi, L.,Canada, F.J.,Jimenez-Barbero, J.,Benko, M.,Harrach, B.,van Raaij, M.J.
Structure and Sialyllactose Binding of the Carboxy-Terminal Head Domain of the Fibre from a Siadenovirus, Turkey Adenovirus 3.
PLoS ONE, 10:e0139339-e0139339, 2015

PubMed Abstract: The virulent form of turkey adenovirus 3 (TAdV-3), also known as turkey hemorrhagic enteritis virus (THEV), is an economically important poultry pathogen, while the avirulent form is used as a vaccine. TAdV-3 belongs to the genus Siadenovirus. The carboxy-terminal region of its fibre does not have significant sequence similarity to any other adenovirus fibre heads of known structure. Two amino acid sequence differences between virulent and avirulent TAdV-3 map on the fibre head: where virulent TAdV-3 contains Ile354 and Thr376, avirulent TAdV-3 contains Met354 and Met376. We determined the crystal structures of the trimeric virulent and avirulent TAdV-3 fibre head domains at 2.2 Å resolution. Each monomer contains a beta-sandwich, which, surprisingly, resembles reovirus fibre head more than other adenovirus fibres, although the ABCJ-GHID topology is conserved in all. A beta-hairpin insertion in the C-strand of each trimer subunit embraces its neighbouring monomer. The avirulent and virulent TAdV-3 fibre heads are identical apart from the exact orientation of the beta-hairpin insertion. In vitro, sialyllactose was identified as a ligand by glycan microarray analysis, nuclear magnetic resonance spectroscopy, and crystallography. Its dissociation constant was measured to be in the mM range by isothermal titration calorimetry. The ligand binds to the side of the fibre head, involving amino acids Glu392, Thr419, Val420, Lys421, Asn422, and Gly423 binding to the sialic acid group. It binds slightly more strongly to the avirulent form. We propose that, in vivo, the TAdV-3 fibre may bind a sialic acid-containing cell surface component.

PubMed: 26418008
DOI: 10.1371/journal.pone.0139339

実験手法

X-RAY DIFFRACTION (2.2 Å)