3ZNY

Crystal structure of the class A extended-spectrum beta-lactamase CTX- M-96, a natural D240G mutant derived from CTX-M-12

3ZNY の概要

• エントリーDOI: 10.2210/pdb3zny/pdb
• 関連するPDBエントリー: 3ZNT 3ZNW
• 分子名称: CTX-M-12A ENZYME (2 entities in total)
• 機能のキーワード: hydrolase, oxyimino-cephalosporinase, ceftazidimase
• 由来する生物種: KLEBSIELLA PNEUMONIAE
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28125.83

構造登録者

Power, P.,Herman, R.,Bouillenne, F.,Ghiglione, B.,Rodriguez, M.M.,Galleni, M.,Gutkind, G.,Charlier, P.,Sauvage, E. (登録日: 2013-02-18, 公開日: 2014-01-08, 最終更新日: 2023-12-20)

主引用文献

Ghiglione, B.,Rodriguez, M.M.,Herman, R.,Curto, L.M.,Dropa, M.,Bouillenne, F.,Kerff, F.,Galleni, M.,Charlier, P.,Gutkind, G.,Sauvage, E.,Power, P.
Structural and Kinetic Insights Into the "Ceftazidimase" Behavior of the Extended-Spectrum Beta-Lactamase Ctx-M-96.
Biochemistry, 54:5072-, 2015

PubMed Abstract: Diversification of the CTX-M β-lactamases led to the emergence of variants responsible for decreased susceptibility to ceftazidime, like the Asp240Gly-harboring "ceftazidimases". We solved the crystallographic structure of the Asp240Gly variant CTX-M-96 at 1.2 Å and evaluated the role of Asp240 in the activity toward oxyimino-cephalosporins through simulated models and kinetics. There seem to be subtle changes in the conformation of the active site cavity of CTX-M-96, compared to enzyme variants harboring the Asp240, and these small rearrangements could be due to localized shifts in the environment of the β3 strand. According to the crystallographic evidence, CTX-M-96 presents a "compact" active site, which in spite of its reduced cavity seems to allow the proper interaction with oxyimino-cephalosporins, as suggested by simulated models. The term "ceftazidimases" that is currently applied for the Asp240Gly-harboring CTX-M variants should be used carefully. Structural differences between CTX-M harboring the Asp240Gly mutation (and also probably others like those at Pro167) do not seem to be conclusive to determine the "ceftazidimase" behavior observed in vivo, which is in turn partially supported by the mild improvement in the catalytic efficiency toward ceftazidime by CTX-M-96 and similar enzymes, compared to "parental" Asp240-harboring variants. In addition, it is observed that alterations in OmpF expression could act synergistically with CTX-M-96 for yielding clinical resistance toward ceftazidime. We therefore propose that the observed resistance in vivo is due to the sum of synergic mechanisms, and the term "cefotaximases associated with ceftazidime resistance" could be conveniently used to describe CTX-M harboring the Asp240Gly substitution.

PubMed: 26228623
DOI: 10.1021/ACS.BIOCHEM.5B00313

実験手法

X-RAY DIFFRACTION (1.2 Å)