3ZMR

Bacteroides ovatus GH5 xyloglucanase in complex with a XXXG heptasaccharide

3ZMR の概要

• エントリーDOI: 10.2210/pdb3zmr/pdb
• 分子名称: CELLULASE (GLYCOSYL HYDROLASE FAMILY 5), alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-D-glucose, ... (7 entities in total)
• 機能のキーワード: hydrolase, xyloglucan
• 由来する生物種: BACTEROIDES OVATUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 109237.34

構造登録者

Larsbrink, J.,Rogers, T.E.,Hemsworth, G.R.,McKee, L.S.,Spadiut, O.,Klinter, S.,Pudlo, N.A.,Urs, K.,Kelly, A.G.,Cederholm, S.N.,Davies, G.J.,Martens, E.C.,Brumer, H. (登録日: 2013-02-12, 公開日: 2014-01-15, 最終更新日: 2023-12-20)

主引用文献

Larsbrink, J.,Rogers, T.E.,Hemsworth, G.R.,Mckee, L.S.,Tauzin, A.S.,Spadiut, O.,Klinter, S.,Pudlo, N.A.,Urs, K.,Koropatkin, N.M.,Creagh, A.L.,Haynes, C.A.,Kelly, A.G.,Cederholm, S.N.,Davies, G.J.,Martens, E.C.,Brumer, H.
A Discrete Genetic Locus Confers Xyloglucan Metabolism in Select Human Gut Bacteroidetes
Nature, 506:498-, 2014

PubMed Abstract: A well-balanced human diet includes a significant intake of non-starch polysaccharides, collectively termed 'dietary fibre', from the cell walls of diverse fruits and vegetables. Owing to the paucity of alimentary enzymes encoded by the human genome, our ability to derive energy from dietary fibre depends on the saccharification and fermentation of complex carbohydrates by the massive microbial community residing in our distal gut. The xyloglucans (XyGs) are a ubiquitous family of highly branched plant cell wall polysaccharides whose mechanism(s) of degradation in the human gut and consequent importance in nutrition have been unclear. Here we demonstrate that a single, complex gene locus in Bacteroides ovatus confers XyG catabolism in this common colonic symbiont. Through targeted gene disruption, biochemical analysis of all predicted glycoside hydrolases and carbohydrate-binding proteins, and three-dimensional structural determination of the vanguard endo-xyloglucanase, we reveal the molecular mechanisms through which XyGs are hydrolysed to component monosaccharides for further metabolism. We also observe that orthologous XyG utilization loci (XyGULs) serve as genetic markers of XyG catabolism in Bacteroidetes, that XyGULs are restricted to a limited number of phylogenetically diverse strains, and that XyGULs are ubiquitous in surveyed human metagenomes. Our findings reveal that the metabolism of even highly abundant components of dietary fibre may be mediated by niche species, which has immediate fundamental and practical implications for gut symbiont population ecology in the context of human diet, nutrition and health.

PubMed: 24463512
DOI: 10.1038/NATURE12907

実験手法

X-RAY DIFFRACTION (1.43 Å)