3ZLC

Crystal Structure of Erv41p

3ZLC の概要

• エントリーDOI: 10.2210/pdb3zlc/pdb
• 分子名称: ER-DERIVED VESICLES PROTEIN ERV41 (2 entities in total)
• 機能のキーワード: protein transport, early secretory pathway, endoplasmic reticulum, copii vesicle
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Endoplasmic reticulum membrane; Multi-pass membrane protein: Q04651
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58219.30

構造登録者

Biterova, E.I.,Svard, M.,Possner, D.D.D.,Guy, J.E. (登録日: 2013-01-30, 公開日: 2013-03-27, 最終更新日: 2024-11-06)

主引用文献

Biterova, E.I.,Svard, M.,Possner, D.D.D.,Guy, J.E.
The Crystal Structure of the Lumenal Domain of Erv41P, a Protein Involved in Transport between the Endoplasmic Reticulum and Golgi Apparatus
J.Mol.Biol., 425:2208-, 2013

PubMed Abstract: Erv41p is a conserved integral membrane protein that is known to play a role in transport between the endoplasmic reticulum and Golgi apparatus, part of the early secretory pathway of eukaryotes. However, the exact function of the protein is not known, and it shares very low sequence identity with proteins of known structure or function. Here we present the structure of the full lumenal domain of Erv41p from Saccharomyces cerevisiae, determined by X-ray crystallography to a resolution of 2.0Å. The structure reveals the protein to be composed predominantly of two large β-sheets that form a twisted β-sandwich. Comparison to structures in the Protein Data Bank shows that the Erv41p lumenal domain displays only limited similarity to β-sandwich domains of other proteins. Analysis of the surface properties of the protein identifies an extensive patch of negative electrostatic potential on the exposed surface of one of the β-sheets, which likely forms a binding site for a ligand or interaction partner. A predominantly hydrophobic region close to the membrane interface is identified as a likely site for protein-protein interaction. This structure, the first of Erv41p or any of its homologues, provides a new starting point for studies of the roles of Erv41p and its interaction partners in the eukaryotic secretory pathway.

PubMed: 23524136
DOI: 10.1016/J.JMB.2013.03.024

実験手法

X-RAY DIFFRACTION (1.999 Å)