3ZKO
The structure of ''breathing'' dengue virus.
Summary for 3ZKO
| Entry DOI | 10.2210/pdb3zko/pdb |
| EMDB information | 2296 |
| Descriptor | ENVELOPE GLYCOPROTEIN (1 entity in total) |
| Functional Keywords | virus, breathing structure, dynamic structure |
| Biological source | DENGUE VIRUS 2 |
| Cellular location | Virion membrane ; Multi-pass membrane protein : Q66394 |
| Total number of polymer chains | 3 |
| Total formula weight | 163205.27 |
| Authors | Fibriansah, G.,Ng, T.S.,Kostyuchenko, V.A.,Lee, S.,Wang, J.,Lok, S.M. (deposition date: 2013-01-24, release date: 2013-05-15, Last modification date: 2024-05-08) |
| Primary citation | Fibriansah, G.,Ng, T.S.,Kostyuchenko, V.A.,Lee, J.,Lee, S.,Wang, J.,Lok, S.M. Structural Changes of Dengue Virus When Exposed to 37 Degrees C. J.Virol., 87:7585-, 2013 Cited by PubMed Abstract: Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This was confirmed by our cryo-electron microscopy images of dengue virus incubated at 37°C, where viruses change their surface from smooth to rough. Here we present the cryo-electron microscopy structures of dengue virus at 37°C. Image analysis showed four classes of particles. The three-dimensional (3D) map of one of these classes, representing half of the imaged virus population, shows that the E protein shell has expanded and there is a hole at the 3-fold vertices. Fitting E protein structures into the map suggests that all of the interdimeric and some intradimeric E protein interactions are weakened. The accessibility of some previously found cryptic epitopes on this class of particles is discussed. PubMed: 23637405DOI: 10.1128/JVI.00757-13 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (13.7 Å) |
Structure validation
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