3ZJ1

Structure of Nab2p tandem zinc finger 12

3ZJ1 の概要

• エントリーDOI: 10.2210/pdb3zj1/pdb
• 関連するPDBエントリー: 3ZJ2
• NMR情報: BMRB: 18955
• 分子名称: NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, ZINC ION (2 entities in total)
• 機能のキーワード: rna binding protein, ccch-type zinc finger, mrna export, poly(a) length control, polyadenosine rna binding.
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Nucleus: P32505
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9726.93

構造登録者

Martinez-Lumbreras, S.,Santiveri, C.M.,Mirassou, Y.,Zorrilla, S.,Perez-Canadillas, J.M. (登録日: 2013-01-16, 公開日: 2013-09-11, 最終更新日: 2024-05-15)

主引用文献

Martinez-Lumbreras, S.,Santiveri, C.M.,Mirassou, Y.,Zorrilla, S.,Perez-Canadillas, J.M.
Two Singular Types of Ccch Tandem Zinc Finger in Nab2P Contribute to Polyadenosine RNA Recognition.
Structure, 21:1800-, 2013

PubMed Abstract: The seven C-terminal CCCH-type zinc fingers of Nab2p bind the poly(A) tail of mRNA (∼A25). Using NMR, we demonstrated that the first four (Zf1-Zf4) contain two structurally independent tandems (TZF12 and TZF34) and bind A12 with moderate affinity (KD = 2.3 μM). Nab2p TZF12 contains a long α helix that contacts the zinc fingers Zf1 and Zf2 to arrange them similarly to Zf6-7 in the Nab2p Zf5-7 structure. Nab2p TZF34 exhibits a distinctive two-fold symmetry of the zinc centers with mutual recognition of histidine ligands. Our mutagenesis and NMR data demonstrate that the α helix of TZF12 and Zf3 of TZF34 define the RNA-binding interface, while Zf1, Zf2, and Zf4 seem to be excluded. These results further our understanding of polyadenosine RNA recognition by the CCCH domain of Nab2p. Moreover, we describe a hypothetical mechanism for controlling poly(A) tail length with specific roles for TZF12, TZF34, and Zf5-7 domains.

PubMed: 23994011
DOI: 10.1016/J.STR.2013.07.019

実験手法

SOLUTION NMR