3ZIF

Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly

3ZIF の概要

• エントリーDOI: 10.2210/pdb3zif/pdb
• EMDBエントリー: 2273
• 分子名称: HEXON PROTEIN, PENTON PROTEIN, PIX, ... (4 entities in total)
• 機能のキーワード: virus, assembly intermediate
• 由来する生物種: BOVINE ADENOVIRUS 3; 詳細
• 細胞内の位置: Virion : P03278
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 1371535.73

構造登録者

Cheng, L.,Huang, X.,Li, X.,Xiong, W.,Sun, W.,Yang, C.,Zhang, K.,Wang, Y.,Liu, H.,Ji, G.,Sun, F.,Zheng, C.,Zhu, P. (登録日: 2013-01-09, 公開日: 2014-01-22, 最終更新日: 2024-05-08)

主引用文献

Cheng, L.,Huang, X.,Li, X.,Xiong, W.,Sun, W.,Yang, C.,Zhang, K.,Wang, Y.,Liu, H.,Huang, X.,Ji, G.,Sun, F.,Zheng, C.,Zhu, P.
Cryo-Em Structures of Two Bovine Adenovirus Type 3 Intermediates
Virology, 450:174-, 2014

PubMed Abstract: Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical. The overall facet structure of BAd3 exhibits a similar structure to human Ads; however, BAd3 protein IX has a unique conformation. Mass spectrometry and cryo-electron tomography analyses indicate that one intermediate structure represents the stage during DNA encapsidation, whilst the other intermediate structure represents a later stage. These results also suggest that cleavage of precursor protein VI occurs during, rather than after, the DNA encapsidation process. Overall, our results provide insights into the mechanism of Ad assembly, and allow the first structural comparison between human and nonhuman Ads at backbone level.

PubMed: 24503080
DOI: 10.1016/J.VIROL.2013.12.012

実験手法

ELECTRON MICROSCOPY (4.5 Å)