3ZGI

Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP

3ZGI の概要

• エントリーDOI: 10.2210/pdb3zgi/pdb
• 関連するPDBエントリー: 3ZGH
• 分子名称: CELL WALL SURFACE ANCHOR FAMILY PROTEIN, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: structural protein, adhesin, keratin-10, srrp
• 由来する生物種: STREPTOCOCCUS PNEUMONIAE
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 67619.44

構造登録者

Schulte, T.,Loefling, J.,Mikaelsson, C.,Kikhney, A.,Hentrich, K.,Diamante, A.,Ebel, C.,Normark, S.,Svergun, D.,Henriques-Normark, B.,Achour, A. (登録日: 2012-12-17, 公開日: 2013-12-25, 最終更新日: 2024-10-16)

主引用文献

Schulte, T.,Lofling, J.,Mikaelsson, C.,Kikhney, A.,Hentrich, K.,Diamante, A.,Ebel, C.,Normark, S.,Svergun, D.,Henriques-Normark, B.,Achour, A.
The Basic Keratin 10-Binding Domain of the Virulence-Associated Pneumococcal Serine-Rich Protein Psrp Adopts a Novel Mscramm Fold.
Open Biol., 4:0090-, 2014

PubMed Abstract: Streptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-rich repeat protein (PsrP) is a lung-specific virulence factor whose functional binding region (BR) binds to keratin-10 (KRT10) and promotes pneumococcal biofilm formation through self-oligomerization. We present the crystal structure of the KRT10-binding domain of PsrP (BR187-385) determined to 2.0 Å resolution. BR187-385 adopts a novel variant of the DEv-IgG fold, typical for microbial surface components recognizing adhesive matrix molecules adhesins, despite very low sequence identity. An extended β-sheet on one side of the compressed, two-sided barrel presents a basic groove that possibly binds to the acidic helical rod domain of KRT10. Our study also demonstrates the importance of the other side of the barrel, formed by extensive well-ordered loops and stabilized by short β-strands, for interaction with KRT10.

PubMed: 24430336
DOI: 10.1098/RSOB.130090

実験手法

X-RAY DIFFRACTION (2.25 Å)