3ZEH

Solution structure of the Hs. PSIP1 PWWP domain

Summary for 3ZEH

• Entry DOI: 10.2210/pdb3zeh/pdb
• NMR Information: BMRB: 18878
• Descriptor: PC4 AND SFRS1-INTERACTING PROTEIN (1 entity in total)
• Functional Keywords: dna binding
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Nucleus: O75475
• Total number of polymer chains: 1
• Total formula weight: 11903.59

Authors

van Ingen, H.,van Nuland, R.,Timmers, H.T.M.,Boelens, R. (deposition date: 2012-12-05, release date: 2013-05-22, Last modification date: 2024-06-19)

Primary citation

Van Nuland, R.,Van Schaik, F.M.,Simonis, M.,Van Heesch, S.,Cuppen, E.,Boelens, R.,Timmers, H.T.M.,Van Ingen, H.
Nucleosomal DNA Binding Drives the Recognition of H3K36-Methylated Nucleosomes by the Psip1-Pwwp Domain.
Epigenetics Chromatin, 6:12-, 2013

PubMed Abstract: Recognition of histone modifications by specialized protein domains is a key step in the regulation of DNA-mediated processes like gene transcription. The structural basis of these interactions is usually studied using histone peptide models, neglecting the nucleosomal context. Here, we provide the structural and thermodynamic basis for the recognition of H3K36-methylated (H3K36me) nucleosomes by the PSIP1-PWWP domain, based on extensive mutational analysis, advanced nuclear magnetic resonance (NMR), and computational approaches.

PubMed: 23656834
DOI: 10.1186/1756-8935-6-12

Experimental method

SOLUTION NMR