3ZEH

Solution structure of the Hs. PSIP1 PWWP domain

3ZEH の概要

• エントリーDOI: 10.2210/pdb3zeh/pdb
• NMR情報: BMRB: 18878
• 分子名称: PC4 AND SFRS1-INTERACTING PROTEIN (1 entity in total)
• 機能のキーワード: dna binding
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus: O75475
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11903.59

構造登録者

van Ingen, H.,van Nuland, R.,Timmers, H.T.M.,Boelens, R. (登録日: 2012-12-05, 公開日: 2013-05-22, 最終更新日: 2024-06-19)

主引用文献

Van Nuland, R.,Van Schaik, F.M.,Simonis, M.,Van Heesch, S.,Cuppen, E.,Boelens, R.,Timmers, H.T.M.,Van Ingen, H.
Nucleosomal DNA Binding Drives the Recognition of H3K36-Methylated Nucleosomes by the Psip1-Pwwp Domain.
Epigenetics Chromatin, 6:12-, 2013

PubMed Abstract: Recognition of histone modifications by specialized protein domains is a key step in the regulation of DNA-mediated processes like gene transcription. The structural basis of these interactions is usually studied using histone peptide models, neglecting the nucleosomal context. Here, we provide the structural and thermodynamic basis for the recognition of H3K36-methylated (H3K36me) nucleosomes by the PSIP1-PWWP domain, based on extensive mutational analysis, advanced nuclear magnetic resonance (NMR), and computational approaches.

PubMed: 23656834
DOI: 10.1186/1756-8935-6-12

実験手法

SOLUTION NMR