3ZBQ

Protofilament of TubZ from Bacteriophage PhiKZ

Summary for 3ZBQ

• Entry DOI: 10.2210/pdb3zbq/pdb
• Related: 3ZBP
• Descriptor: PHIKZ039, GUANOSINE-5'-DIPHOSPHATE (3 entities in total)
• Functional Keywords: viral protein, ftsz, tubulin, tubz, cytoskeleton
• Biological source: PSEUDOMONAS PHAGE PHIKZ
• Total number of polymer chains: 1
• Total formula weight: 37744.16

Authors

Aylett, C.H.S.,Lowe, J. (deposition date: 2012-11-12, release date: 2013-04-03, Last modification date: 2023-12-20)

Primary citation

Aylett, C.H.S.,Izore, T.,Amos, L.A.,Lowe, J.
Structure of the Tubulin/Ftsz-Like Protein Tubz from Pseudomonas Bacteriophage Phikz
J.Mol.Biol., 425:2164-, 2013

PubMed Abstract: Pseudomonas ΦKZ-like bacteriophages encode a group of related tubulin/FtsZ-like proteins believed to be essential for the correct centring of replicated bacteriophage virions within the bacterial host. In this study, we present crystal structures of the tubulin/FtsZ-like protein TubZ from Pseudomonas bacteriophage ΦKZ in both the monomeric and protofilament states, revealing that ΦKZ TubZ undergoes structural changes required to polymerise, forming a canonical tubulin/FtsZ-like protofilament. Combining our structures with previous work, we propose a polymerisation-depolymerisation cycle for the Pseudomonas bacteriophage subgroup of tubulin/FtsZ-like proteins. Electron cryo-microscopy of ΦKZ TubZ filaments polymerised in vitro implies a long-pitch helical arrangement for the constituent protofilaments. Intriguingly, this feature is shared by the other known subgroup of bacteriophage tubulin/FtsZ-like proteins from Clostridium species, which are thought to be involved in partitioning the genomes of bacteriophages adopting a pseudo-lysogenic life cycle.

PubMed: 23528827
DOI: 10.1016/J.JMB.2013.03.019

Experimental method

X-RAY DIFFRACTION (1.7 Å)