3ZBI

Fitting result in the O-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase

3ZBI の概要

• エントリーDOI: 10.2210/pdb3zbi/pdb
• 関連するPDBエントリー: 3ZBJ
• EMDBエントリー: 2233
• 分子名称: TRAF PROTEIN, TRAO PROTEIN, TRAN PROTEIN (3 entities in total)
• 機能のキーワード: cell adhesion, bacterial secretion
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 598907.04

構造登録者

Rivera-Calzada, A.,Fronzes, R.,Savva, C.G.,Chandran, V.,Lian, P.W.,Laeremans, T.,Pardon, E.,Steyaert, J.,Remaut, H.,Waksman, G.,Orlova, E.V. (登録日: 2012-11-10, 公開日: 2013-04-03, 最終更新日: 2024-05-08)

主引用文献

Rivera-Calzada, A.,Fronzes, R.,Savva, C.G.,Chandran, V.,Lian, P.W.,Laeremans, T.,Pardon, E.,Steyaert, J.,Remaut, H.,Waksman, G.,Orlova, E.V.
Structure of a Bacterial Type Iv Secretion Core Complex at Subnanometre Resolution.
Embo J., 32:1195-, 2013

PubMed Abstract: Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

PubMed: 23511972
DOI: 10.1038/EMBOJ.2013.58

実験手法

ELECTRON MICROSCOPY (8.5 Å)