3X2O

Neutron and X-ray joint refined structure of PcCel45A apo form at 298K.

3X2O の概要

• エントリーDOI: 10.2210/pdb3x2o/pdb
• 関連するPDBエントリー: 3X2l 3x2g 3x2h 3x2i 3x2j 3x2k 3x2m 3x2n 3x2p
• 分子名称: Endoglucanase V-like protein (2 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Phanerochaete chrysosporium (White-rot fungus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18178.79

構造登録者

Nakamura, A.,Ishida, T.,Kusaka, K.,Yamada, T.,Tanaka, I.,Niimura, N.,Samejima, M.,Igarashi, K. (登録日: 2014-12-22, 公開日: 2015-10-07, 最終更新日: 2019-12-18)

主引用文献

Nakamura, A.,Ishida, T.,Kusaka, K.,Yamada, T.,Fushinobu, S.,Tanaka, I.,Kaneko, S.,Ohta, K.,Tanaka, H.,Inaka, K.,Higuchi, Y.,Niimura, N.,Samejima, M.,Igarashi, K.
"Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography.
Sci Adv, 1:e1500263-e1500263, 2015

PubMed Abstract: Hydrolysis of carbohydrates is a major bioreaction in nature, catalyzed by glycoside hydrolases (GHs). We used neutron diffraction and high-resolution x-ray diffraction analyses to investigate the hydrogen bond network in inverting cellulase PcCel45A, which is an endoglucanase belonging to subfamily C of GH family 45, isolated from the basidiomycete Phanerochaete chrysosporium. Examination of the enzyme and enzyme-ligand structures indicates a key role of multiple tautomerizations of asparagine residues and peptide bonds, which are finally connected to the other catalytic residue via typical side-chain hydrogen bonds, in forming the "Newton's cradle"-like proton relay pathway of the catalytic cycle. Amide-imidic acid tautomerization of asparagine has not been taken into account in recent molecular dynamics simulations of not only cellulases but also general enzyme catalysis, and it may be necessary to reconsider our interpretation of many enzymatic reactions.

PubMed: 26601228
DOI: 10.1126/sciadv.1500263

実験手法

NEUTRON DIFFRACTION (1.5 Å)
X-RAY DIFFRACTION (1 Å)