3X0Y

Crystal structure of FMN-bound DszC from Rhodococcus erythropolis D-1

Summary for 3X0Y

• Entry DOI: 10.2210/pdb3x0y/pdb
• Related: 3X0X
• Descriptor: DszC, FLAVIN MONONUCLEOTIDE (3 entities in total)
• Functional Keywords: dbt monooxygenase, desulfurization, acyl-coa dehydrogenase domain, fmn-dependent, oxidoreductase
• Biological source: Rhodococcus erythropolis
• Total number of polymer chains: 8
• Total formula weight: 362985.56

Authors

Guan, L.J.,Lee, W.C.,Wang, S.P.,Ohtsuka, J.,Tanokura, M. (deposition date: 2014-10-23, release date: 2015-02-25, Last modification date: 2024-05-29)

Primary citation

Guan, L.J.,Lee, W.C.,Wang, S.,Ohshiro, T.,Izumi, Y.,Ohtsuka, J.,Tanokura, M.
Crystal structures of apo-DszC and FMN-bound DszC from Rhodococcus erythropolis D-1.
Febs J., 282:3126-3135, 2015

PubMed Abstract: The release of SO2 from petroleum products derived from crude oil, which contains sulfur compounds such as dibenzothiophene (DBT), leads to air pollution. The '4S' metabolic pathway catalyzes the sequential conversion of DBT to 2-hydroxybiphenyl via three enzymes encoded by the dsz operon in several bacterial species. DszC (DBT monooxygenase), from Rhodococcus erythropolis D-1 is involved in the first two steps of the '4S' pathway. Here, we determined the first crystal structure of FMN-bound DszC, and found that two distinct conformations occur in the loop region (residues 131-142) adjacent to the active site. On the basis of the DszC-FMN structure and the previously reported apo structures of DszC homologs, the binding site for DBT and DBT sulfoxide is proposed.

PubMed: 25627402
DOI: 10.1111/febs.13216

Experimental method

X-RAY DIFFRACTION (2.3 Å)