3X0Y

Crystal structure of FMN-bound DszC from Rhodococcus erythropolis D-1

3X0Y の概要

• エントリーDOI: 10.2210/pdb3x0y/pdb
• 関連するPDBエントリー: 3X0X
• 分子名称: DszC, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: dbt monooxygenase, desulfurization, acyl-coa dehydrogenase domain, fmn-dependent, oxidoreductase
• 由来する生物種: Rhodococcus erythropolis
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 362985.56

構造登録者

Guan, L.J.,Lee, W.C.,Wang, S.P.,Ohtsuka, J.,Tanokura, M. (登録日: 2014-10-23, 公開日: 2015-02-25, 最終更新日: 2024-05-29)

主引用文献

Guan, L.J.,Lee, W.C.,Wang, S.,Ohshiro, T.,Izumi, Y.,Ohtsuka, J.,Tanokura, M.
Crystal structures of apo-DszC and FMN-bound DszC from Rhodococcus erythropolis D-1.
Febs J., 282:3126-3135, 2015

PubMed Abstract: The release of SO2 from petroleum products derived from crude oil, which contains sulfur compounds such as dibenzothiophene (DBT), leads to air pollution. The '4S' metabolic pathway catalyzes the sequential conversion of DBT to 2-hydroxybiphenyl via three enzymes encoded by the dsz operon in several bacterial species. DszC (DBT monooxygenase), from Rhodococcus erythropolis D-1 is involved in the first two steps of the '4S' pathway. Here, we determined the first crystal structure of FMN-bound DszC, and found that two distinct conformations occur in the loop region (residues 131-142) adjacent to the active site. On the basis of the DszC-FMN structure and the previously reported apo structures of DszC homologs, the binding site for DBT and DBT sulfoxide is proposed.

PubMed: 25627402
DOI: 10.1111/febs.13216

実験手法

X-RAY DIFFRACTION (2.3 Å)