3X0T

Crystal structure of PirA

3X0T の概要

• エントリーDOI: 10.2210/pdb3x0t/pdb
• 関連するPDBエントリー: 3X0U
• 分子名称: Uncharacterized protein, NITRATE ION (3 entities in total)
• 機能のキーワード: jelly roll fold, pore forming toxin, toxin
• 由来する生物種: Vibrio parahaemolyticus M0605
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27892.35

構造登録者

Wang, H.C.,Ko, T.P.,Wang, A.H.J.,Lo, C.F. (登録日: 2014-10-22, 公開日: 2015-08-26, 最終更新日: 2024-05-29)

主引用文献

Lee, C.T.,Chen, I.T.,Yang, Y.T.,Ko, T.P.,Huang, Y.T.,Huang, J.Y.,Huang, M.F.,Lin, S.J.,Chen, C.Y.,Lin, S.S.,Lin, S.S.,Lightner, D.V.,Wang, H.C.,Wang, A.H.,Wang, H.C.,Hor, L.I.,Lo, C.F.
The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin.
Proc.Natl.Acad.Sci.USA, 112:10798-10803, 2015

PubMed Abstract: Acute hepatopancreatic necrosis disease (AHPND) is a severe, newly emergent penaeid shrimp disease caused by Vibrio parahaemolyticus that has already led to tremendous losses in the cultured shrimp industry. Until now, its disease-causing mechanism has remained unclear. Here we show that an AHPND-causing strain of V. parahaemolyticus contains a 70-kbp plasmid (pVA1) with a postsegregational killing system, and that the ability to cause disease is abolished by the natural absence or experimental deletion of the plasmid-encoded homologs of the Photorhabdus insect-related (Pir) toxins PirA and PirB. We determined the crystal structure of the V. parahaemolyticus PirA and PirB (PirA(vp) and PirB(vp)) proteins and found that the overall structural topology of PirA(vp)/PirB(vp) is very similar to that of the Bacillus Cry insecticidal toxin-like proteins, despite the low sequence identity (<10%). This structural similarity suggests that the putative PirAB(vp) heterodimer might emulate the functional domains of the Cry protein, and in particular its pore-forming activity. The gene organization of pVA1 further suggested that pirAB(vp) may be lost or acquired by horizontal gene transfer via transposition or homologous recombination.

PubMed: 26261348
DOI: 10.1073/pnas.1503129112

実験手法

X-RAY DIFFRACTION (1.17 Å)