3WYO

Heterodimeric myoglobin formed by domain swapping

3WYO の概要

• エントリーDOI: 10.2210/pdb3wyo/pdb
• 関連するPDBエントリー: 3VM9
• 分子名称: Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: globin, oxygen binding
• 由来する生物種: Equus caballus (horse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 70400.00

構造登録者

Lin, Y.W.,Nagao, S.,Zhang, M.,Shomura, Y.,Higuchi, Y.,Hirota, S. (登録日: 2014-09-04, 公開日: 2014-11-19, 最終更新日: 2023-11-08)

主引用文献

Lin, Y.W.,Nagao, S.,Zhang, M.,Shomura, Y.,Higuchi, Y.,Hirota, S.
Rational design of heterodimeric protein using domain swapping for myoglobin.
Angew.Chem.Int.Ed.Engl., 54:511-515, 2015

PubMed Abstract: Protein design is a useful method to create novel artificial proteins. A rational approach to design a heterodimeric protein using domain swapping for horse myoglobin (Mb) was developed. As confirmed by X-ray crystallographic analysis, a heterodimeric Mb with two different active sites was produced efficiently from two surface mutants of Mb, in which the charges of two amino acids involved in the dimer salt bridges were reversed in each mutant individually, with the active site of one mutant modified. This study shows that the method of constructing heterodimeric Mb with domain swapping is useful for designing artificial multiheme proteins.

PubMed: 25370865
DOI: 10.1002/anie.201409267

実験手法

X-RAY DIFFRACTION (2 Å)