3WX7

Crystal structure of COD

Summary for 3WX7

• Entry DOI: 10.2210/pdb3wx7/pdb
• Descriptor: Chitin oligosaccharide deacetylase, CALCIUM ION, ZINC ION, ... (6 entities in total)
• Functional Keywords: oligosaccharide deacetylace, carbohydrate-binding, hydrolase
• Biological source: Vibrio parahaemolyticus
• Total number of polymer chains: 2
• Total formula weight: 89506.75

Authors

Park, S.-Y.,Sugiyama, K.,Hirano, T.,Nishio, T. (deposition date: 2014-07-18, release date: 2014-12-03, Last modification date: 2024-10-30)

Primary citation

Hirano, T.,Sugiyama, K.,Sakaki, Y.,Hakamata, W.,Park, S.Y.,Nishio, T.
Structure-based analysis of domain function of chitin oligosaccharide deacetylase from Vibrio parahaemolyticus.
FEBS Lett., 589:145-151, 2015

PubMed Abstract: The X-ray crystal structure of chitin oligosaccharide deacetylase from Vibrio parahaemolyticus (Vp-COD) was determined at an 1.35 Å resolution. The amino acid sequence and structure of Vp-COD show that the enzyme comprises one polysaccharide deacetylase domain (PDD) and two carbohydrate-binding domains (CBDs). On the basis of a chitin-binding assay with Vp-COD and its CBDs-deleted mutant, it was confirmed that CBDs can adhere to chitin. The catalytic activity of the CBDs-deleted mutant was only mildly depressed compared with that of Vp-COD, indicating that CBDs are unlikely to affect the configuration of the active center residues in active site of PDD.

PubMed: 25479092
DOI: 10.1016/j.febslet.2014.11.039

Experimental method

X-RAY DIFFRACTION (1.349 Å)