3WWF

Crystal structure of the computationally designed Pizza2-SR protein

3WWF の概要

• エントリーDOI: 10.2210/pdb3wwf/pdb
• 関連するPDBエントリー: 3WW7 3WW8 3WW9 3WWA 3WWB
• 分子名称: pizza2sr-pb protein (2 entities in total)
• 機能のキーワード: computational protein design, self-assembly, de novo protein
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 27429.33

構造登録者

Voet, A.R.D.,Noguchi, H.,Addy, C.,Simoncini, D.,Terada, D.,Unzai, S.,Park, S.Y.,Zhang, K.Y.J.,Tame, J.R.H. (登録日: 2014-06-17, 公開日: 2014-10-08, 最終更新日: 2024-03-20)

主引用文献

Voet, A.R.D.,Noguchi, H.,Addy, C.,Simoncini, D.,Terada, D.,Unzai, S.,Park, S.Y.,Zhang, K.Y.J.,Tame, J.R.H.
Computational design of a self-assembling symmetrical beta-propeller protein.
Proc.Natl.Acad.Sci.USA, 111:15102-15107, 2014

PubMed Abstract: The modular structure of many protein families, such as β-propeller proteins, strongly implies that duplication played an important role in their evolution, leading to highly symmetrical intermediate forms. Previous attempts to create perfectly symmetrical propeller proteins have failed, however. We have therefore developed a new and rapid computational approach to design such proteins. As a test case, we have created a sixfold symmetrical β-propeller protein and experimentally validated the structure using X-ray crystallography. Each blade consists of 42 residues. Proteins carrying 2-10 identical blades were also expressed and purified. Two or three tandem blades assemble to recreate the highly stable sixfold symmetrical architecture, consistent with the duplication and fusion theory. The other proteins produce different monodisperse complexes, up to 42 blades (180 kDa) in size, which self-assemble according to simple symmetry rules. Our procedure is suitable for creating nano-building blocks from different protein templates of desired symmetry.

PubMed: 25288768
DOI: 10.1073/pnas.1412768111

実験手法

X-RAY DIFFRACTION (1.601 Å)