3WV7

HcgE from Methanothermobacter marburgensis

3WV7 の概要

• エントリーDOI: 10.2210/pdb3wv7/pdb
• 関連するPDBエントリー: 3WV8 3WV9 3WVA 3WVB 3WVC
• 分子名称: Hmd co-occurring protein HcgE, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: e1 enzyme superfamily, uba/thif-type nad/fad binding fold, adenylyltransferase, atp binding, transferase
• 由来する生物種: Methanothermobacter marburgensis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 71642.00

構造登録者

Fujishiro, T.,Ermler, U.,Shima, S. (登録日: 2014-05-16, 公開日: 2015-04-29, 最終更新日: 2023-11-08)

主引用文献

Fujishiro, T.,Kahnt, J.,Ermler, U.,Shima, S.
Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor
Nat Commun, 6:6895-6895, 2015

PubMed Abstract: The iron-guanylylpyridinol (FeGP) cofactor of [Fe]-hydrogenase contains a prominent iron centre with an acyl-Fe bond and is the only acyl-organometallic iron compound found in nature. Here, we identify the functions of HcgE and HcgF, involved in the biosynthesis of the FeGP cofactor using structure-to-function strategy. Analysis of the HcgE and HcgF crystal structures with and without bound substrates suggest that HcgE catalyses the adenylylation of the carboxy group of guanylylpyridinol (GP) to afford AMP-GP, and subsequently HcgF catalyses the transesterification of AMP-GP to afford a Cys (HcgF)-S-GP thioester. Both enzymatic reactions are confirmed by in vitro assays. The structural data also offer plausible catalytic mechanisms. This strategy of thioester activation corresponds to that used for ubiquitin activation, a key event in the regulation of multiple cellular processes. It further implicates a nucleophilic attack onto the acyl carbon presumably via an electron-rich Fe(0)- or Fe(I)-carbonyl complex in the Fe-acyl formation.

PubMed: 25882909
DOI: 10.1038/ncomms7895

実験手法

X-RAY DIFFRACTION (1.6 Å)