3WUI

Dimeric horse cytochrome c formed by refolding from molten globule state

3WUI の概要

• エントリーDOI: 10.2210/pdb3wui/pdb
• 関連するPDBエントリー: 3WC8
• 分子名称: Cytochrome c, HEME C, TETRAETHYLENE GLYCOL, ... (6 entities in total)
• 機能のキーワード: electron transport
• 由来する生物種: Equus caballus (horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12845.54

構造登録者

Deshpande, M.S.,Parui, P.P.,Kamikubo, H.,Yamanaka, M.,Nagao, S.,Komori, H.,Kataoka, M.,Higuchi, Y.,Hirota, S. (登録日: 2014-04-25, 公開日: 2014-07-16, 最終更新日: 2024-11-20)

主引用文献

Deshpande, M.S.,Parui, P.P.,Kamikubo, H.,Yamanaka, M.,Nagao, S.,Komori, H.,Kataoka, M.,Higuchi, Y.,Hirota, S.
Formation of domain-swapped oligomer of cytochrome C from its molten globule state oligomer.
Biochemistry, 53:4696-4703, 2014

PubMed Abstract: Many proteins, including cytochrome c (cyt c), have been shown to form domain-swapped oligomers, but the factors governing the oligomerization process remain unrevealed. We obtained oligomers of cyt c by refolding cyt c from its acid molten globule state to neutral pH state under high protein and ion concentrations. The amount of oligomeric cyt c obtained depended on the nature of the anion (chaotropic or kosmotropic) in the solution: ClO4(-) (oligomers, 11% ± 2% (heme unit)), SCN(-) (10% ± 2%), I(-) (6% ± 2%), NO3(-) (3% ± 1%), Br(-) (2% ± 1%), Cl(-) (2% ± 1%), and SO4(2-) (3% ± 1%) for refolding of 2 mM cyt c (anion concentration 125 mM). Dimeric cyt c obtained by refolding from the molten globule state exhibited a domain-swapped structure, in which the C-terminal α-helices were exchanged between protomers. According to small-angle X-ray scattering measurements, approximately 25% of the cyt c molecules were dimerized in the molten globule state containing 125 mM ClO4(-). These results indicate that a certain amount of molten globule state oligomers of cyt c convert to domain-swapped oligomers during refolding and that the intermolecular interactions necessary for domain swapping are present in the molten globule state.

PubMed: 24981551
DOI: 10.1021/bi500497s

実験手法

X-RAY DIFFRACTION (1.8 Å)